陕西省犊牛安氏隐孢子虫多位点序列分型研究

doi:10.11843/j.issn.0366-6964.2017.10.020

Abstract

Abstract:

To study the multilocus sequence types of Cryptosporidium andersoni from calves in Shaanxi Province, 36 fecal samples positive for C. andersoni were collected from 0-6 months calves in four regions of Shaanxi province and examined by the polymerase chain reaction (PCR). Sequence analysis was based on four genetic loci, namely CM-MS1, CM-MS2, CM-MS3 and CM-MS16. The results showed that a total of 15 C. andersoni isolates were successfully genotyped in all four loci and divided into 4 subtypes of MLST, namely A4, A4, A2, A1; A4, A4, A4, A1; A5, A4, A4, A1; A1, A4, A4, A1. Among them, the MLST (A4, A4, A4, A1), as the dominant subtype, was widely distributed in various farms and different breeds of calves. These findings indicated that genetic variation was presented in C. andersoni of calves in Shaanxi province and various MLST subtypes were found in these calves.

CLC Number:

S852.723

REN Guan-jing, WANG Xue-ting, ZHANG Hui-jun, SONG Jun-ke, ZHAO Guang-hui. Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1969-1975.

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Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province

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